Nexcelom Bioscience Cellometer Auto 2000 Cell Profiler Counter

Description

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Nexcelom Bioscience Cellometer Auto 2000 Cell Profiler Counter
Description
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Description
Shipping
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Medical Alert!
Lease/Financing
Nexcelom Bioscience Cellometer Auto 2000 Cell Profiler Counter
**Unit has been tested for basic functionality. Has slight cosmetic blemishes from normal use. See photos for further details.
The Cellometer Auto 2000 has been particularly optimized to analyze primary cells from bone marrow, cord blood, peripheral blood, and other complex samples for use in various research areas, such as:
Suspensions of tumor cells for oncology
Stem cells for cellular therapy
Splenocytes for development of vaccines
PBMCs for immunology
Nucleated cells for transplantation
Both live and dead nucleated cells can be stained with dual-color fluorescence. This produces precise viability results even in the presence of red blood cells, platelets, and debris. Precise analysis of both “clean” and “messy” samples allows the Cellometer Auto 2000 to assess samples at many different points throughout sample processing, right from initial collection through to separation and cryopreservation.
The Cellometer Auto 2000 includes one-touch assays for inspecting a variety of primary samples, such as:
The Cellometer Auto 2000 cell viability counter uses dual-fluorescence imaging and bright-field imaging to rapidly and precisely detect and count individual cells. Count, diameter, concentration, and % viability of cells are calculated and reported automatically. Simple, Automated Cell Counting in 30 Seconds.
Load sample, view image, count cells, and obtain results in less than 30 seconds.
The Auto 2000 enables users to:
Enhance consistency
Increase precision
Increase throughput
Avoid judgment errors, interference from red blood cells, miscounts, and user-to-user variability
Count complex cells (both irregular-shaped and clumpy)
Guarantee that all data is properly captured
-Nucleated Cells from Whole Blood
(Immune Cells, high RBC)
-
Stem Cells
(BR/Green/Red, Stem Cells)
-
PBMCs/Splenocytes Following Separation
(Immune Cells,Low RBC)
-
Tumor and Tissue Cell Suspension
(Primary Cells, Cell Lines)
No Interference from Red Blood Cells, Platelets, or Debris
The dual-fluorescence AO/PI technique uses nuclear staining dyes that adhere to nucleic acids in the cell nucleus. Since a majority of the mature mammalian red blood cells lack nuclei, only live mononuclear cells and dead mononuclear cells create a fluorescent signal. This means, red blood cells do not have to be lysed, which saves time and prevents the additional sample preparation step. Debris, platelets, and red blood cells are not counted in the fluorescent channels.
The Advantage of Fluorescent Counting For Primary Cells
The following images reveal the benefit of fluorescent counting for primary cells. The bright-field image demonstrates the combination of platelets, red blood cells, and nucleated cells present in the sample. Only the live nucleated cells and dead nucleated cells are observed and counted in the red and green fluorescent channels.
Fresh human peripheral blood mononuclear cells (PBMCs) were spiked with different amounts of red blood cells. All cells (both RBC and nucleated) were counted in the bright-field channel. Subsequently, nucleated cells were counted in the green fluorescent channel. Different amounts of red blood cells (1.8%, 4.6%, and 8.9%) did not have any impact on the nucleated cell count.
User-Friendly Touch Screen
The easy-to-use touch screen shows pre-optimized assays for normal types of cells. User-defined protocols can be easily produced and saved to the menu. The pre-optimized and simple assays make it easy to operate the instrument and streamline training for novice users.
Cell Size Analysis and Size-Based Counting;
It takes about 5 minutes to count 1 x 10
6
cells with a manual hemacytometer. At times, it takes twice the time to count live cells and dead cells. The Cellometer Auto 2000 Cell Viability Counter estimates the cell count and concentration for both dead and live cells and also calculates % viability in only 30 seconds.
Cellometer Precision
The Cellometer Auto 2000 cell counter provides exceptional reproducibility, with a % coefficient of variation (CV) of less than 10% for fluorescent concentration as well as viability measurements.
The following data is based on four preparations of Jurkat cells stained with a fluorescent nuclear-staining dye called propidium iodide.
The Cellometer Disposable Imaging Chambers contain a pair of separately enclosed chambers with an accurately controlled height. A 20 µL cell suspension is placed into the chamber using a regular single-channel pipette. This chamber, in turn, is embedded into the Cellometer cell counter and the cells are finally imaged.
This method of sample loading and analysis is simple and suitable for fragile cells.
The disposable Cellometer cell counting chambers provides many major benefits:
Risk of cross-contamination is eliminated
The washing step is eliminated, which saves time
A controlled volume of samples is achieved
Decreased biohazard risk to users
The most affordable automated counting consumables
Large-depth chambers for huge cells
SKU: 3364564
We take great care packaging every item to ensure safe shipment to you. We use top quality packaging materials and equipment.
We DO NOT allow buyers to arrange their own shipping. However, we don't charge a fee for arranging the shipping for you. The cost of pallets or crates necessary for freight shipping may be extra. All orders are automatically shipped with added insurance that covers the FULL value of your purchase.
Standard Domestic Shipping
Standard shipping methods are chosen for you at our discretion, either by USPS, FedEx or UPS. Tracking numbers are emailed automatically at the end of the business day, once your shipment has left our facility. UPS overnight is only available until 12pm PST in order for us to ship on the same day. Please call to verify we've received your overnight request. Same day shipping overnight is not available during holidays or weekends. If you are sending to a PO Box, your purchase must be shipped via USPS, either First Class, Priority, or Parcel Post, without exception.
Freight
For larger and sensitive items that we have designated for freight shipment, please contact us for a shipping quote before you pay online. Items over 70 pounds will likely ship on a pallet or crate, whose costs will be the buyer's responsibility. Freight shipments will require a quote before you can make payment. Please contact us with:
Your shipping address
Confirm if your address is commercial or residential
Inform us if you require a lift gate
Failure to provide this information could result in extra fees assessed by the carrier and we may bill you for the difference.
Damage Shipments
If your item arrives damaged by the shipping carrier, please take clear images before & after opening your package to document the extent of the damage. The claim will require pictures of different angles of the box/crate before opening, and the damaged product(s) after opening. Email us immediately with your order number and item description, along with the images and we'll begin the claim process for you. We manage you claim at no extra cost. However, failure to provide adequate and timely information may result in a claim being denied, which will affect our ability to offer a refund.
Free Pick up
Will-call is available for those arranging local pick ups. Commercial carriers do not qualify as local pick up. We do not charge an extra fee if you want to pick your item up. The item will only be available as a loose load and will not be prepared for shipping. If you require a pallet for transport a fee may apply.
Special Packaging
We can have custom ISO certified heat-treated wood pallets, skids and crates built, if necessary. We do not charge a palletizing or packaging fee, but if your item requires a pallet, skid or crate, we will invoice you for the added costs.
International shipping
International orders will need to contact us and ask for a shipping quote. All International customers are responsible for their country's duties & taxes. Please check with your country's requirements before purchasing.
We do offer international freight shipments. Simply contact us for a freight shipment quote according to the regular freight shipment instructions.
International Shipping Restrictions
In order to be eligible for standard international shipment, items must have a final sale price of at least 0. In order to be eligible for international freight shipments, items must have a final sale price of ,500.
We do not offer international shipping to countries restricted by United States law.
Deductible
We are happy to arrange shipping for you, we DO NOT charge an extra fee for this service. When using us to arrange your shipping your item(s) are automatically shipped with added insurance that covers the FULL value of your purchase; you will be responsible for a 10% deductible.
Genuinely faulty goods will be replaced or money refunded, however we must be advised of any faulty goods upon receipt of the item. It is the customer's responsibility to pay for and ship the item back to us. If the item is found to be faulty, we will also refund the shipping costs.
If you are unsatisfied with your purchase in any way, please contact us first so we can rectify the situation immediately. You do not need to open a case on ebay or leave negative feedback, your complete satisfaction is more than enough motivation for us to do whatever it takes to make things right!
Please call our toll free number on our web site
Monday − Friday, 9am to 6pm PST
We bring integrity to the on-line market place. At REUZEit, customer service comes natural and is part of our core values. We have extensive knowledge about many common machines, if we do not know the answer right away, we will find out.
30-Day, 100% No Hassle, warranty
All our products carry a 30-Day Warranty, unless specifically stated otherwise. If the product does not work you may return it for a replacement or refund within 30 days of our ship date. Customer is responsible for return shipping and item's safe arrival at our facility.
If you receive a non-functional product, please contact us to hold a replacement or return the item with your order number. All items must be returned in exact condition received, including any accessories & packaging when applicable.
We accept:
Visa, MasterCard, Discover and American Express
PayPal
Company checks (must cash before shipping)
Wire transfers are encouraged and required for any international orders
ACH transfers
Cash on pick up
Customer Service
Open: Monday-Friday 9AM-6PM PST
REUZEit, LLC
28381 Vincent Moraga Dr
3rd Floor
Temecula, CA 92590
Phone: Please visit our web page for toll free phone number
The sale of this item may be subject to regulation by the U.S. Food and Drug Administration and state and local regulatory agencies. If so, do not bid on this item unless you are an authorized purchaser. If the item is subject to FDA regulation, an REUZEit representative will verify your status as an authorized purchaser of this item before shipping of the item.
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